Phytoplankton pigment concentrations measured by HPLC in the surface water during 4 expeditions from spring to fall in 2019, Mackenzie Delta Region (CA)

The determination of phytoplankton using high performance liquid chromatography (HPLC) is detailed in Hooker et al. (2005; doi:10.1364/AO.44.000553) and Ras et al. (2008; doi:10.5194/bg-5-353-2008). Briefly, the pigments of particles retained on GF/F (0.7 µm) filters were extracted at -20°C with 3 mL methanol (100%). The filter samples were then disrupted using a sonicator and clarified one hour later by vacuum filtration through GF/F filters. The extracts were rapidly analyzed within 24h by HPLC with a complete Agilent Technologies system (comprising LC Chemstation software, a degasser, a binary pump, a refrigerated autosampler, a column thermostat and a diode array detector).

Data and Resources

Additional Info

Field Value
Identifier DOI:10.1594/PANGAEA.937585
Project(s) NUNATARYUK, Permafrost thaw and the changing Arctic coast, science for socioeconomic adaptation
Institute Takuvik International Research Laboratory (IRL 3376), ULaval – CNRS, Biology department, Laval University, Quebec, Canada
Source https://doi.org/10.1594/PANGAEA.937585
Publication Date 2021-10-27
Version 1.0
Product
Sensor High Performance Liquid Chromatography (HPLC)
Files
  1. NunaWP4Mackenzie19_phytopl_pigments TAB delimited text file
Variables [Units]
  1. Event
  2. Expedition
  3. Latitude
  4. Longitude
  5. Date/Time
  6. Station
  7. Depth water [m]
  8. Chl a: Chlorophyll a [mg/m³]
  9. Chl b: Chlorophyll b [mg/m³]
  10. Chl c: Chlorophyll c [mg/m³]
  11. a-Car + b-Car: alpha-Carotene + beta-Carotene [mg/m³]
  12. But-fuco: 19-Butanoyloxyfucoxanthin [ mg/m³]
  13. Hex-fuco: 19-Hexanoyloxyfucoxanthin [mg/m³]
  14. Allo: Alloxanthin [mg/m³]
  15. Diadino: Diadinoxanthin [mg/m³]
  16. Diato: Diatoxanthin [mg/m³]
  17. Fuco: Fucoxanthin [mg/m³]
  18. Perid: Peridinin [mg/m³]
  19. Zea: Zeaxanthin [mg/m³]
  20. MV chl a: Monovinyl chlorophyll a [mg/m³]
  21. Chlide a: Chlorophyllide a [mg/m³]
  22. MV chl b: Monovinyl chlorophyll b [mg/m³]
  23. Chl c1+c2+Mg DVP: Chlorophyll c2 + chlorophyll c1 + Mg-2,4-divinyl pheoporphyrin [mg/m³]
  24. Chl c3: Chlorophyll c3 [mg/m³]
  25. Lut: Lutein [mg/m³]
  26. Neo: Neoxanthin [mg/m³]
  27. Viola: Violaxanthin [mg/m³]
  28. Phytin a tot: Pheophytin a, total [mg/m³]
  29. Phide a tot: Pheophorbide a, total [mg/m³]
  30. Pras: Prasinoxanthin [mg/m³]
  31. Chl tot: Chlorophyll total [mg/m³]
  32. PPC: Carotenoid pigments, photoprotective [mg/m³]
  33. PSC: Carotenoid pigments, photosynthetic [mg/m³]
  34. PSP: Pigments, photosynthetic [mg/m³]
  35. Carotenoids tot: Carotenoids, total [mg/m³]
  36. Pigm tot acc: Pigments, total accessory [mg/m³]
  37. Pigm tot: Pigments, total [mg/m³]
  38. Pigm tot diag: Pigments, total diagnostic [mg/m³]
  39. Pigm acc/chl a: Pigments, accessory/chlorophyll a ratio
  40. PSC/Carotenoids tot: Carotenoid pigments, photosynthetic/Carotenoids, total ratio
  41. PPC/Carotenoids tot: Carotenoid pigments, photoprotective/Carotenoids, total ratio
  42. Chl tot/Carotenoids to: Chlorophyll, total/Carotenoids, total ratio
  43. PPC/Pigm tot: Carotenoid pigments, photoprotective/Pigments, total
  44. PSC/Pigm tot: Carotenoid pigments, photosynthetic/Pigments, total
  45. Chl a/Pigm tot: Chlorophyll a/Pigments, total
Region Mackenzie Delta Region
Spatial Reference EPSG:4326 WGS 84
Spatial Resolution
Spatial Coverage Latitude 68.264050 to 69.648520, Longitude -138.135120 to -133.031410
Temporal Coverage 2019
Temporal Resolution April - September
Format TXT
Is Supplement To

Juhls, B., Lizotte, M., et al. (2021). Hydrographical, biogeochemical and bio-optical water properties in the Mackenzie Delta Region during 4 expeditions from spring to fall in 2019, https://doi.pangaea.de/10.1594/PANGAEA.937587
Related to

Lizotte, M. Juhls, B. et al. (2023). Nunataryuk field campaigns: Understanding the origin and fate of terrestrial organic matter in the coastal waters of the Mackenzie Delta region. Earth System Science data. Earth Syst. Sci. Data, 15, 1617–1653. https://doi.org/10.5194/essd-15-1617-2023

Juhls, B, Matsuoka, A., Lizotte, M. et al. (2022). Seasonal dynamics of dissolved organic matter in the Mackenzie Delta, Canadian Arctic waters: Implications for ocean colour remote sensing. Remote Sensing of Environment, 283,113327. https://doi.org/10.1016/j.rse.2022.113327.

Dataset extent