Anaerobic incubation measurements (CH4+CO2) and microbe quantification, Lena Delta, 2018

Laurent, Mélissa; Fuchs, Matthias; Treat, Claire C; Liebner, Susanne; Runge, Alexandra

The data sets were made during the summer 2021, with samples collected from three cores, at two depths (active and permafrost layers). In total, six samples (3 replicates by samples) were incubated for 67 days at two temperatures (4°C and 20°C). Core sampling were performed during the joint Russian-German LENA 2018 expedition. The data sets were both collected at Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research and GeoForschungsZentrum Helmholtz-Zentrum, Potsdam, Germany. The aim of this study was to understand and quantify how much carbon may be lost during short-term permafrost thaw across different landscape units at the example of study area in the Lena Delta, Siberia. The study measures greenhouse gases (GHG) emissions based on an incubation experiment and focuses on relationships between GHG emissions and microbial abundance shifts during short-term permafrost thaw under anaerobic conditions. The objectives of the study were to: (1) Quantify CH4 and CO2 production during a short-term anaerobic incubation; (2) Establish relationships between CH4 and CO2 production and microbes (methanogens and methanotrophs); (3) and to identify settings and controls that drive gas production rates in thawed permafrost soils.

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Contact

Laurent, Mélissa

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Papildoma informacija

Laukas Reikšmė
Identifier DOI: 10.1594/PANGAEA.945685
Project(s) The role of non-growing season processes in the methane and nitrous oxide budgets in pristine northern ecosystems (FluxWIN)
Institute Alfred Wegener Institute for Polar and Marine Science
Šaltinis https://doi.org/10.1594/PANGAEA.945685
Publication Date 2023
Versija 1.0
Product CO2 and CH4 production data and microbial quantification
Sensor Bio-Rad Laboratories CFX96 thermal cycler; Agilent Technologies 7890A GC
Files
  1. DNA_qPCR_Laurent_etal-2022.tab
  2. Inc_CH4_CO2_Laurent_etal-2022.tab
Variables [Units]
  1. Event: Event label
  2. Sample label: Sample code/label
  3. Latitude: [°N]
  4. Longitude: [°E]
  5. Date/Time: Date and time
  6. Location: Location
  7. Country: Country
  8. site: Site ID
  9. Vegetation type: Vegetation Type
  10. Ecosystem: Ecosystem
  11. Samp pos: Sample position
  12. Soil type: Soil Type
  13. Samp type: Sample Type
  14. State: State of Permafrost
  15. Inc dur: Incubation duration [days]
  16. T incubation: Time incubation [days]
  17. Inc Temp: Incubation Temperature [°C]
  18. Repl: Replicates [#]
  19. Depth top: Depth top/min [m]
  20. Depth bot: Depth bottom/max [m]
  21. Depth sed: Depth sediment/rock [m]
  22. Exp treat: Experimental Treatment
  23. DNA conc: DNA Concentration [ng/µl]
  24. V: Volume [µl]
  25. Dilution: Dilution Factor
  26. pmoA: Methane oxygenase, pmoA gene, abundance
  27. mcrA: Methyl coenzyme M reductase, mcrA gene, abundance
  28. Veg cov: Vegetation, cover
  29. Soil class: Soil classification system
  30. Thaw depth: Thaw depth of active layer [cm]
  31. Depth desc: Depth description
  32. TOC: Total Organic Carbon [%]
  33. TC: Total Carbon [%]
  34. TN: Total Nitrogen [%]
  35. C/N: Carbon to Nitrogen ratio [mol/mol]
  36. VWC: Volumetric Water Content [%]
  37. pH: pH
  38. BD perma: Bulk Density [g/cm3]
  39. CH4-C/C rate: Methane, production rate per mass carbon [µg/g/day]
  40. CH4-C/soil rate: Methane, production rate per mass soil [µg/g/day]
  41. CO2-C/C rate: Carbon dioxide, production rate per mass carbon [µg/g/day]
  42. CO2-C/soil rate: Carbon dioxide, production rate per mass soil [µg/g/day]
Region Lena River Delta
Spatial Reference
Spatial Resolution Point based
Spatial Coverage Latitude: 72.356000, Longitude: 126.314647
Temporal Coverage 2018-08-13
Temporal Resolution Discrete, Daily
Formatas TXT
Is Supplement To

Laurent, M., Fuchs, M., Herbst, T., Runge, A., Liebner, S., and Treat, C. C.: Relationships between greenhouse gas production and landscape position during short-term permafrost thaw under anaerobic conditions in the Lena Delta, Biogeosciences, 20, 2049–2064, https://doi.org/10.5194/bg-20-2049-2023, 2023.
Related to

Thauer, Rudolf K (1998): Biochemistry of methanogenesis: a tribute to Marjory Stephenson:1998 Marjory Stephenson Prize Lecture. Microbiology, 144(9), 2377-2406, https://doi.org/10.1099/00221287-144-9-2377

Theisen, Andreas R; Murrell, Michael T (2005): Facultative Methanotrophs Revisited. Journal of Bacteriology, 187(13), 4303-4305, https://doi.org/10.1128/JB.187.13.4303-4305.2005

Robertson, G Philip; Coleman, David C; Bledsoe, Caroline S; Sollins, Phillip (2009): Standard Soil Methods for Long-Term Ecological Research. Oxford University Press

Dataset extent